Some feedback from Gerrit Botha at UCT CBIO: A hands-on course in 16S microbial composition analysis recently held at UCT and the HPC cluster was used as a training platform. Participants received a temporary account and most of the exercises were done using an interactive qsub session. The QIIME (Quantitative Insights Into Microbial Ecology) tool set was used for data cleaning and preliminary analysis while the R phyloseq package was used for final statistical analysis. The 200 series was sufficient for processing the small test datasets, however real datasets are larger and will have memory requirements in the range of the 400 series. The overall course feedback was positive and the UCT participants plan to register for permanent accounts to start processing their own data. The only part that proved challenging was to get the participants accustomed to the Linux environment. Many of the them had no or limited exposure to Linux so future courses will definitely need to keep it in mind and give a thorough introduction to the environment.